Definition of ExopeptidaseExopeptidase: An enzyme that catalyzes the cleavage of the terminal (last) or next-to-last peptide bond from a polypeptide or protein, releasing a single amino acid or dipeptide. By contrast, an endopeptidase catalyzes the cleavage of then internal peptide bonds within a polypeptide or protein. Last Editorial Review: 6/14/2012
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